|
Status |
Public on Jul 11, 2008 |
Title |
Gene expression profile of MEF cells transformed by the two H-RasV12 and Twist2 oncogenes |
Sample type |
RNA |
|
|
Source name |
Primary MEF cells transformed by H-RasV12 and Twist2
|
Organism |
Mus musculus |
Characteristics |
MEF cells from C57/B16 x DBA2 F1 mice
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA form primary or Ras + Twist-transformed MEF cells were prepared in duplicates using an RNeasy Mini kit (Quiagen).
|
Label |
Biotin
|
Label protocol |
Amplification and biotin-labeling were performed according to the Message Amp kit II (Ambion).
|
|
|
Hybridization protocol |
Codelink Expression Assay protocol
|
Scan protocol |
Codelink Expression Assay protocol
|
Description |
control group
|
Data processing |
Codelink Expression analysis v4.2 software
|
|
|
Submission date |
Jun 12, 2008 |
Last update date |
Jun 12, 2008 |
Contact name |
stéphane ansieau |
Organization name |
Inserm U590
|
Street address |
28 rue laennec
|
City |
Lyon |
ZIP/Postal code |
69008 |
Country |
France |
|
|
Platform ID |
GPL2897 |
Series (1) |
GSE11756 |
Comparison of primary MEF vs HRasV12 + Twist transformed MEF |
|