|
| Status |
Public on Jun 12, 2008 |
| Title |
Control_No.2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
REF
|
| Organism |
Drosophila melanogaster |
| Characteristics |
RNA from cells grown from 0.5 million/ml for 48hr. without ecdysone treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen) and mRNA was prepared with oligo-dT cellulose (Ambion).
|
| Label |
CY3
|
| Label protocol |
The mRNA was labeled with ith Cy3/5 using SuperScript (Invitrogen).
|
| |
|
| Channel 2 |
| Source name |
EXP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
RNA from cells grown from 0.5 million/ml for 48hr. and then grow for another 48 hours without ecdysone treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen) and mRNA was prepared with oligo-dT cellulose (Ambion).
|
| Label |
CY5
|
| Label protocol |
The mRNA was labeled with ith Cy3/5 using SuperScript (Invitrogen).
|
| |
|
| |
| Hybridization protocol |
As described in Sun, L.V. etal PNAS 2003
|
| Scan protocol |
As described in Sun, L.V. etal PNAS 2003
|
| Description |
cells were grown from 0.5 million/ml for 48hr., start from here (start point 0hr), grow cells for another 48hr (no ecdysone treatment), compare expression profile of these untreated cell to untreated cells at 0hr.
|
| Data processing |
Data processing VALUE is log2 (CY5/CY3; exp/ref)
|
| |
|
| Submission date |
May 29, 2008 |
| Last update date |
Jun 11, 2008 |
| Contact name |
Zareen Gauhar |
| E-mail(s) |
[email protected]
|
| Organization name |
Yale
|
| Department |
Genetics
|
| Lab |
White Lab
|
| Street address |
333 Cedar St, 386 NSB
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platform ID |
GPL6912 |
| Series (1) |
| GSE11625 |
Ecdysone Response Timecourse in Kc Cells |
|
