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Sample GSM2936143 Query DataSets for GSM2936143
Status Public on Jan 17, 2018
Title ESC_D01_RNA-seq
Sample type SRA
 
Source name Embryonic stem cell NMT-seq
Organism Mus musculus
Characteristics cell line: El16 embyonic stem cell
strain: 129xCast/129
Growth protocol ESCs were cultured in serum/LIF conditions as described previously (Ficz et al., Cell Stem Cell 13, 351–359)
Extracted molecule polyA RNA
Extraction protocol DNA and mRNA were seperately purified from single cells using the G&T-seq protocol described previously (Macaulay et al., Nature Protocols, 2016)
Libraries were prepared using the G&T-seq protocol described previously (Macaulay et al., Nature Protocols 2016)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description Single-cell RNA-seq
ESC_RNA_counts.txt.gz
Data processing Libraries were sequenced on the Illumina HiSeq or NextSeq platform using the default RTA analysis software.
Raw sequences were trimmed with Trim Galore (v0.4.3; Cutadapt v1.9.1) and aligned to the GRCm38 genome with HISAT2 (v2.0.5; options --dta --sp 1000,1000 to disallow soft-clipping). Gene expression counts were quantified from the mapped reads using featureCounts within the R package Rsubread. Gene annotations were obtained from Ensembl version 87. Only protein-coding genes matching canonical chromosomes were considered.
genome build: GRCm38
Supplementary_files_format_and_content: RNA-Seq reports contain raw counts per gene, with one row per gene (Ensembl Gene ID) and one column for each sample.
 
Submission date Jan 16, 2018
Last update date Jan 24, 2018
Contact name Felix Krueger
E-mail(s) [email protected]
Organization name Altos Labs
Department Bioinformatics
Street address Granta Park
City Cambridge
ZIP/Postal code CB21 6GP
Country United Kingdom
 
Platform ID GPL13112
Series (1)
GSE109262 Joint profiling of chromatin accessibility, DNA methylation and transcription in single cells
Relations
BioSample SAMN08368258
SRA SRX3585553

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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