|
| Status |
Public on Mar 30, 2018 |
| Title |
MM231 cells tfxn LSD1 shRNA, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
cell type: breast cancer cell line
transfected with: LSD1 shRNA
|
| Treatment protocol |
shRNA Lentiviral Particles were purchased from Santa Cruz Biotecnology and transduced according to manufacturer's protocol (http://www.scbt.com/protocols.html?protocol=shrna_lentiviral_particles_transduction)
|
| Growth protocol |
normal culturing conditions for MDA-MB-231
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAgen RNeasy Mini (Animal Cells Spin protocol, w/DNase I digest performed on homogenized lysate prior to applying lysate to column for purification)
|
| Label |
biotin
|
| Label protocol |
cRNA synthesis and labeling was performed using the Ambion MessageAmp Premier Kit (Life Technologies)
|
| |
|
| Hybridization protocol |
cRNA was hybridized to U133A 2.0 arrays (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
cRNA synthesis and labeling, hybridization, and scanning were performed by the University of Pittsburgh Cancer Biomarkers Facility
|
| Description |
MDA-MB-231 cells, LSD1 shRNA transfection, biological replicate 2
|
| Data processing |
The data is RMA normalized using 'affy' package in R-Bioconductor
|
| |
|
| Submission date |
Jan 03, 2018 |
| Last update date |
Mar 30, 2018 |
| Contact name |
Yi Huang |
| Organization name |
University of Pittsburgh Cancer Institute
|
| Department |
Pharmacology & Chemical Biology
|
| Lab |
MWRI, B430I/J
|
| Street address |
204 Craft Ave
|
| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE72687 |
RNA expression in MDA-MB-231 cells transfected with scramble, LSD1 or HDAC5 shRNA (HG-U133A_2) |
|
