|
| Status |
Public on Feb 16, 2018 |
| Title |
BACE457_40Tun_100 |
| Sample type |
RNA |
| |
|
| Source name |
HEK-293 (human embryonic kidney)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK-293 (human embryonic kidney)
treatment: 40 ng/ml Tun
er stress-inducing drug: yes
expressing model polypeptides: no
|
| Growth protocol |
in vitro
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufacturer's recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyzer).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Whole Genome Gene Expression Direct Hybridization
|
| Scan protocol |
Illumina HiScan
|
| Description |
BACE457
|
| Data processing |
The data were normalised using quantile method
|
| |
|
| Submission date |
Dec 20, 2017 |
| Last update date |
Feb 16, 2018 |
| Contact name |
Andrea Rinaldi |
| E-mail(s) |
[email protected]
|
| Phone |
918200385
|
| Organization name |
IOR - oncology institute of southern switzerland
|
| Street address |
via vela 6
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE108346 |
Comparison between drugs- and unfolded proteins-induced responses reveals early induction of components of an ER-resident multimeric complex binding unfolded proteins |
|
