|
| Status |
Public on Mar 17, 2018 |
| Title |
Stretched_NRVMs_control_rep_02b |
| Sample type |
RNA |
| |
|
| Source name |
Neonatal rat ventricular myocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
treatment: control
|
| Treatment protocol |
Stretch was introduced to cells by applying a cyclic vacuum suction under the flexible bottomed plates by computer-controlled equipment (Flexercell Strain Unit FX-3000, Flexcell).
|
| Growth protocol |
Neonatal rat ventricular myocytes were prepared from 2-4 days old Sprague-Dawley rats as previously described (Pikkarainen et al.J. Biol. Chem. 278, 23807–23816 (2003).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Tisolated with TRIzol Reagent (Sigma Aldrich) according to the manufacturer’s protocol (Invitrogen) by using the Phase Lock Gel system (Eppendorf).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
Gene expression data from control, non-stretched neonatal rat ventricular myocytes
|
| Data processing |
Robust Multichip Average (RMA) normalization was performed, and quantile normalization, and baseline transformed to median of all samples in GeneSpring 12.6 Software
|
| |
|
| Submission date |
Nov 30, 2017 |
| Last update date |
Mar 17, 2018 |
| Contact name |
Jaana Rysae |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Eastern Finland
|
| Department |
School of Pharmacy
|
| Street address |
Yliopistonranta 1C
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE107551 |
Mechanical stretch induced transcriptomic profiles in cardiac myocytes II |
|
