|
| Status |
Public on Nov 17, 2020 |
| Title |
Cardiomyocytes treated with DOX rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Cardiomyocytes treated with DOX
|
| Organism |
Mus musculus |
| Characteristics |
cell line: HL-1
cell type: Cardiomyocyte cell line
tissue derivation: Heart
treatment: DOX
|
| Treatment protocol |
Mouse cardiomyocytes dispensed in 10ml culture medium were cultured in 10-cm dishes at 80% confluence, DOX (0.3µM) and IFN-γ (20ng/ml) were added.
|
| Growth protocol |
The mouse cardiomyocyte cell line HL-1 was maintained in culture medium consisting of DMEM supplemented with 10% fetal bovine serum at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 250 ng total RNA according to the manufacturer’s standard protocol (Affymetrix).
|
| |
|
| Hybridization protocol |
Following labeling, cDNA was hybridized on a GeneChip®Mouse Transcriptome Array 1.0.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Command Console installed in the GeneChip® Scanner 3000 7G.
|
| Description |
DOX2
Expression data from differently treated mouse cardiomyocytes.
|
| Data processing |
The data was analyzed with the Robust Multichip Analysis (RMA) algorithm, using the Affymetrix default analysis settings.
|
| |
|
| Submission date |
Nov 17, 2017 |
| Last update date |
Nov 17, 2020 |
| Contact name |
Chen Ni |
| E-mail(s) |
[email protected]
|
| Phone |
+86-371-66913632
|
| Organization name |
The First Affiliated Hospital of Zhengzhou University
|
| Department |
Midical Research Center
|
| Street address |
NO.1 Jianshe East Road
|
| City |
ZhengZhou |
| State/province |
Henan |
| ZIP/Postal code |
450052 |
| Country |
China |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE107113 |
Expression data from differently treated mouse cardiomyocytes |
|
