|
| Status |
Public on May 02, 2008 |
| Title |
3hr 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cells treated with estrogen for 3h.
|
| Organism |
Homo sapiens |
| Characteristics |
breast adenocarcinoma from 69yr old female caucasian
|
| Treatment protocol |
MCF-7 cells were stimulated with 100 nM estrogen for 0, 3, 6 or 12 h.
|
| Growth protocol |
MCF-7 cells were maintained and deprived of hormones as previously described in Carroll, J.S. et al. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell 122, 33–43 (2005).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
biological replicate: 3hr 1, 3hr 2, 3hr 3
|
| Data processing |
Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
|
| |
|
| Submission date |
May 02, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
[email protected]
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE11324 |
Genome-wide analysis of estrogen receptor binding sites |
|
| Relations |
| Reanalyzed by |
GSE119087 |
