|
| Status |
Public on Sep 24, 2008 |
| Title |
Control_RNAi_1hr_2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse E16 Hippocampal Neurons, KCl, 1 hr
|
| Organism |
Mus musculus |
| Characteristics |
Mouse E16 Hippocampal Neurons
control RNAi @ 3 DIV
KCl @ 8 DIV
1 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 200 ng total RNA using the Enzo IVT labeling kit (Enzo, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized to the Affymetrix MOE-430-2.0 chip according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneArray Scanner.
|
| Description |
E16 mouse embryonic hippocampal neuron cultures were infected with lentivirus expression shRNA @ 3DIV and depolarized with 55 mM KCl @ 8DIV for 0, 1, 3 and 6 hours.
|
| Data processing |
The data were processed with MAS 5.0. The normalization target was set to 500.
|
| |
|
| Submission date |
Apr 24, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yingxi Lin |
| E-mail(s) |
[email protected]
|
| Phone |
617-355-8395
|
| Organization name |
Children' Hospital/Harvard Medical School
|
| Department |
Neurology/Neurobiology
|
| Lab |
Michael E. Greenberg
|
| Street address |
Enders 250, 300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE11258 |
Npas4-regulated genes in mouse hippocampal neurons |
| GSE11261 |
Study of activity-regulated genes in mouse primary cultured neurons |
|
| Relations |
| Reanalyzed by |
GSE119085 |
