|
| Status |
Public on Aug 01, 2008 |
| Title |
Illini R Green Fruit technical rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
fruit
|
| Organism |
Citrullus lanatus |
| Characteristics |
Watermelon, Cultivar Illini Red
synonym: Citrullus lanatus (Thunb.) Matsum. & Nakai
Field grown
12 days post-pollination
|
| Treatment protocol |
Watermelon fruits at green flesh stage (12 DAP), pink flesh stage (24 DAP), and red flesh stage (36 DAP) from the cultivar ‘Illini Red’ were used for RNA isolation. In addition, several leaves in different stages of development, immature (not fully expanded), young (fully expanded), and mature, were collected from the experimental plants, combined, and processed for RNA isolation.
|
| Growth protocol |
The watermelon plants were grown in a field plot located at the South Central Agricultural Research Laboratory at Lane, OK.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from watermelon fruits and leaves from all bioreps as previously described by Davis et al., HortScience 2006, 41:1292-1294.
|
| Label |
Cy3
|
| Label protocol |
According to standard NimbleGen protocol.
|
| |
|
| Hybridization protocol |
According to standard NimbleGen protocol.
|
| Scan protocol |
According to standard NimbleGen protocol.
|
| Description |
no additional information
|
| Data processing |
Performed by NimbleGen Systems: quantile normalization method of Bolstad et al. (2003). Normalized expression values for the individual probes were used to obtain the expression values for a given open reading frame (ORF) by using the robust multiarray average (RMA) procedure as previously described by Irizarry et al. (2003).
Raw data file (.pair) linked as supplementary file.
|
| |
|
| Submission date |
Apr 23, 2008 |
| Last update date |
Apr 23, 2008 |
| Contact name |
Patrick Wechter |
| E-mail(s) |
[email protected]
|
| Organization name |
USDA-ARS
|
| Department |
U.S. Vegetable Laboratory
|
| Street address |
2700 Savannah Highway
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29414 |
| Country |
USA |
| |
|
| Platform ID |
GPL6772 |
| Series (1) |
| GSE11246 |
Evaluation of genes involved in watermelon fruit ripening and development |
|
