|
| Status |
Public on Jun 17, 2008 |
| Title |
12466 UC Inflamed descending colon |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
UC Inflamed descending colon
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 253
current medication: Asacol 800mg BD
birth date: 3/14/58
ethnicity: CAUCASIAN
symptoms onset date: 8/24/97
diagnosis date: 9/4/97
joint problems: FALSE
uc flare up: FALSE
family history: FALSE
aza treated: unknown
aza tolerant: FALSE
aza at present: FALSE
procedure date: 5/24/05
indictation for procedure:
ucss: 1
calprotectin: 0
esr: 0
crp: 0
hb: 0
wcc: 0
neutrophils: 0
albumin: 0
blood obtained: TRUE
ibd affected relatives: 0
igr2096: 12
igr2198: 12
igr2230: 12
octn1: 12
octn2: 12
nod 702: 11
nod 908: 11
dlg5 133a: 11
nod 1007 fs: 12
diagnosis rectum: BOTH
diagnosis recto sigmoid: BOTH
diagnosis splen flex: BOTH
diagnosis hep flex: BOTH
diagnosis total: BOTH
last followup date: 5/24/05
followup rectum: BOTH
followup recto sigmoid: BOTH
followup splen flex: BOTH
followup hep flex: BOTH
followup total: BOTH
casenote review date: 8/3/05
category: FAILURE OF THERAPY
smoking status: never
smoking start date:
smoking stop date:
smoking amount: unknown
other illnesses: None
disease: UC
run_date: 11-15-01
anatomic_location: descending colon
inflammation_status: Inflamed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA (Catalog #740000)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: total RNA from 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| |
| Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
| Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
| Description |
UC Inflamed descending colon
|
| Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
| |
|
| Submission date |
Apr 21, 2008 |
| Last update date |
Apr 12, 2017 |
| Contact name |
Alex Abbas |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE11223 |
Colon biopsies from UC patients and healthy controls |
|
