|
| Status |
Public on Jun 17, 2008 |
| Title |
11082 Normal Uninflamed descending colon |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Normal Uninflamed descending colon
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 103
current medication: None
birth date: 6/30/65
ethnicity: CAUCASIAN
symptoms onset date:
diagnosis date:
joint problems: FALSE
uc flare up: FALSE
family history: FALSE
aza treated:
aza tolerant: FALSE
aza at present: FALSE
procedure date: 11/9/04
indictation for procedure: Diarrhoea, now settled, Mum has UC
ucss: 0
calprotectin:
esr:
crp:
hb:
wcc:
neutrophils:
albumin:
blood obtained: FALSE
ibd affected relatives:
igr2096:
igr2198:
igr2230:
octn1:
octn2:
nod 702:
nod 908:
dlg5 133a:
nod 1007 fs:
diagnosis rectum: unknown
diagnosis recto sigmoid: unknown
diagnosis splen flex: unknown
diagnosis hep flex: unknown
diagnosis total: unknown
last followup date:
followup rectum: unknown
followup recto sigmoid: unknown
followup splen flex: unknown
followup hep flex: unknown
followup total: unknown
casenote review date:
category: 0
smoking status:
smoking start date:
smoking stop date:
smoking amount:
other illnesses:
disease: Normal
run_date: 8-9-01
anatomic_location: descending colon
inflammation_status: Uninflamed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA (Catalog #740000)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: total RNA from 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| |
| Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
| Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
| Description |
Normal Uninflamed descending colon
|
| Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
| |
|
| Submission date |
Apr 21, 2008 |
| Last update date |
Apr 12, 2017 |
| Contact name |
Alex Abbas |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE11223 |
Colon biopsies from UC patients and healthy controls |
|
| Relations |
| Reanalyzed by |
GSM522148 |
