|
| Status |
Public on Sep 01, 2008 |
| Title |
jejunum_GATA4mutant_2 |
| Sample type |
RNA |
| |
|
| Source name |
jejunum, GATA4 mutant, 2
|
| Organism |
Mus musculus |
| Characteristics |
Gata4Loxp/-VillinCre adult male jejunum (6-8wk). Gata4 conditional knockout (mutant) sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Affymetrix One-cycle target labeling
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Background and Aims: Although the zinc finger transcription factor GATA4 has been implicated in regulating jejunal gene expression, the contribution of GATA4 in controlling jejunal physiology has not been addressed. Methods: We generated mice in which the Gata4 gene was specifically deleted in the small intestinal epithelium. Measurements of plasma cholesterol and phospholipids, intestinal absorption of dietary fat and cholesterol, and gene expression were performed on these animals. Results: Mice lacking GATA4 in the intestine displayed a dramatic block in their ability to absorb cholesterol and dietary fat. Comparison of the global gene expression profiles of control jejunum, control ileum, and GATA4 null jejunum by gene array analysis demonstrated that GATA4 null jejunum lost expression of 53% of the jejunal-specific gene set and gained expression of 47% of the set of genes unique to the ileum. These alterations in gene expression included a decrease in mRNAs encoding lipid and cholesterol transporters as well as an increase in mRNAs encoding proteins involved in bile acid absorption. Conclusion: Our data demonstrate that GATA4 is essential for jejunal function including fat and cholesterol absorption and confirm that GATA4 plays a pivotal role in determining jejunal versus ileal identity.
|
| Data processing |
Standard GCOS data processing produced the values shown.
|
| |
|
| Submission date |
Apr 16, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Stephen A Duncan |
| E-mail(s) |
[email protected]
|
| Phone |
843-792-9104
|
| Organization name |
Medical University South Carolina
|
| Department |
Regenerative Medicine and Cell Biology
|
| Lab |
Duncan Lab
|
| Street address |
173 Ashley Avenue, BSB 6th Floor, Room 657A
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11194 |
GATA4 conditional knockout in the small intestine |
|
| Relations |
| Reanalyzed by |
GSE119085 |
