|
| Status |
Public on Oct 10, 2018 |
| Title |
dendritic cells transfected with negative control - donor 3 |
| Sample type |
RNA |
| |
|
| Source name |
healthy donor 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Dendritic cells
transfection: negative control
|
| Treatment protocol |
After 6 days, 3x106 DCs, obtained from three different healthy donors, were transfected with either miR-29b or NC and co-cultured with U266 MM cells. 24 hours after transfection, cell were harvested and separated with immuno-magnetic microbeads.
|
| Growth protocol |
DCs were generated from healthy donors' CD14+ monocytes isolated by immunomagnetic separation. These cells were then cultured for 6 days in RPMI-1640 medium containing 10% FBS, 1% penicillin/streptomycin, 100 ng/ml recombinant human GM-CSF and 50 ng/ml IL-4 . Half-medium was replaced every 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA (tRNA) from DCs was extracted through column purification with RNeasy kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
cDNA was processed for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix Inc.) according to the Affymetrix’s procedures
|
| |
|
| Hybridization protocol |
cDNA was hybridized on GeneChip® Human Transcriptome Array 2.0 at 45C for 16-18 hours. The arrays were washed and stained on Affymetrix Fluidics Stations 450.
|
| Scan protocol |
The arrays were scanned with an Affymetrix GeneChip Scanner 3000
|
| Description |
gene expression data from dendritic cells transfected with negative control
|
| Data processing |
Data were processed using Affymetrix Expression Console for quality control after summarization and Robust Multi-array Average (RMA) normalization
|
| |
|
| Submission date |
Oct 11, 2017 |
| Last update date |
Oct 10, 2018 |
| Contact name |
Cirino Botta |
| E-mail(s) |
[email protected]
|
| Organization name |
"Annunziata" Hospital of Cosenza
|
| Department |
Hematology Unit
|
| Street address |
Via Migliori
|
| City |
Cosenza |
| ZIP/Postal code |
87100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE104831 |
Transcriptome profiling reveals anti-inflammatory activity of miR-29b in multiple myeloma associated dendritic cells |
|
