|
| Status |
Public on Sep 24, 2008 |
| Title |
mCtx_KCl_1hr_A_1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse P0 cortical neurons, KCl treatment for 1 hour
|
| Organism |
Mus musculus |
| Characteristics |
Mouse P0 cortical neurons
5 DIV, 55 mM KCl treatment for 1 hour
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 200 ng total RNA using the Enzo IVT labeling kit (Enzo, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.7 microg of cRNA were hybridized to the Affymetrix MOE-430A chip according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneArray Scanner.
|
| Description |
Postnatal day 0 mouse embryonic cortical neuron cultures were depolarized with 55 mM KCl for 0, 1 and 6 hours.
|
| Data processing |
The data were processed with MAS 4.0. The normalization target was set to 500.
|
| |
|
| Submission date |
Apr 03, 2008 |
| Last update date |
Sep 24, 2008 |
| Contact name |
Yingxi Lin |
| E-mail(s) |
[email protected]
|
| Phone |
617-355-8395
|
| Organization name |
Children' Hospital/Harvard Medical School
|
| Department |
Neurology/Neurobiology
|
| Lab |
Michael E. Greenberg
|
| Street address |
Enders 250, 300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (2) |
| GSE11256 |
KCl depolarization-regulated genes in mouse cortical neurons |
| GSE11261 |
Study of activity-regulated genes in mouse primary cultured neurons |
|
