|
| Status |
Public on Dec 15, 2017 |
| Title |
Control_660 |
| Sample type |
SRA |
| |
|
| Source name |
LPS induced splenic CD138+ Pb
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
Sex: male
sorting markers: CD138+
strain: C57BL/6J
stimulation: LPS
drug treatment: Tamoxifen
cell type: Plasmablasts
|
| Treatment protocol |
Three days following LPS stimulation, CD138+ cells were magentically enriched from the spleens of wild-type and EZH2-deficient mice.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy Kit.
500ng of total RNA was used as input for the KAPA Biosystems Stranded mRNA-seq kit with polyA selection beads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Ezh2KO.RNAseq.FPKM.mm9.csv
|
| Data processing |
Raw sequencing reads were mapped to the mm9 version of the mouse genome using Tophat2 and the UCSC mm9 known gene reference transcriptome.
Reads mapping to exons were summarized for each unique RefSeq gene using the HOMER package v4.8.2.
Genome_build: mm9
Supplementary_files_format_and_content: Annotation file containing FPKM normalized read counts for each sample.
|
| |
|
| Submission date |
Aug 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chris Scharer |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE103126 |
EZH2 is required for gene repression in Plasmablasts |
| GSE103195 |
Defects in T cell independent B cell differentiation in the absence of EZH2 |
|
| Relations |
| BioSample |
SAMN07561749 |
| SRA |
SRX3135934 |
