|
| Status |
Public on Mar 18, 2008 |
| Title |
hIgG1-treated MDMs_2 days_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
control hlgG1
|
| Organism |
Homo sapiens |
| Characteristics |
MDMs at day 2, biological rep2
|
| Treatment protocol |
Freshly isolated monocytes were treated for 2 days in the presence of 10 ng/ml M-CSF
|
| Growth protocol |
Monocytes were cultured with RPMI 1640 with 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Fragmentation, hybridizatio, washing and staining were performed according Affymetrix's instructions
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| Data processing |
Background correlation, normalization and log2 transformation were carried out using RMA method with their default settings within the Bioconductor suite for R software package
|
| |
|
| Submission date |
Mar 17, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yung-Chi Chang |
| Organization name |
National Yang-Ming University
|
| Department |
Microbiology and Immunology
|
| Street address |
155, Sec. 2, Li-Nong St.
|
| City |
Taipei |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10856 |
Expression data from decoy receptor 3-treated monocyte-derived macrophages |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
