|
| Status |
Public on Mar 13, 2008 |
| Title |
WT Col, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type plants (Col) grown under continuous light, transferrd to the dark for 8h and shifted to light for 2h
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Genotype: Col
Plant material : rosette leaves of 3 week-old plants grown under continuous light 90 mmol. m-2 . s-1, before bolting
|
| Treatment protocol |
Plants were transferred to the dark for 8 h and rosette leaves were harvested 2 h after reillumination.
|
| Growth protocol |
Plants were grown on soil for 3 weeks under continuous light at 90 mmol. m-2 . s-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction was performed according to the guanidine-hydrochloride method as described in Melzer, S., Majewski, D. M. & Apel, K. (1990) Plant Cell 2, 953-961.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Eukaryotic Target Preparation, 2002, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450, protocol EukGE-WS2v4.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from rosette leaves of 3-week-old wild type plants (Ler) grown under continuous light, 2h after a 8hdark/light shift
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 13, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Christophe Laloi |
| E-mail(s) |
[email protected]
|
| Phone |
+41 44 632 63 58
|
| Fax |
+41 44 632 10 81
|
| URL |
http://www.pg.ethz.ch/people/laloic
|
| Organization name |
ETH Zurich
|
| Department |
Institute of Plant Science
|
| Lab |
LFW D17.2
|
| Street address |
Universitaetstrasse 2
|
| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10812 |
Expression data from thylakoidal ascorbate peroxidase overexpressor Arabidopsis thaliana (Col) rosette leaves |
|
| Relations |
| Reanalyzed by |
GSE119083 |
