|
| Status |
Public on Sep 01, 2008 |
| Title |
WT leaf-drought-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
wild type (WT) leaf, drought, replicate 3
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
fully expanded leaves- 7d without water
8 week old plants
|
| Treatment protocol |
Leaf samples (pooled from 10 plants/line) were collected on day 0 and day 7 of the RWC time course and frozen immediately in liquid N2.
|
| Growth protocol |
Wild type (Col 0) and InsP 5-ptase transgenic Arabidopsis plants were grown in the NC State University Phytotron in a growth chamber, under short day conditions (8h light/16 dark) at 21C with a light intensity of ~ 150 μmol m-2 sec-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Plant RNeasy kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated target cRNA was synthesized using the Two cycle Target Labeling protocol (Affymetrix Inc, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Arabidopsis arrays (ATH1 from Affymetrix Inc) were hybridized using the Affymetrix fluidics station.
|
| Scan protocol |
Data acquisition and analysis was carried out by Expression Analysis Inc. (Durham, NC) using the Affymetrix scanner and GCOS software.
|
| Description |
WT leaf-drought-rep3
|
| Data processing |
An estimate of signal for each transcript was calculated based on the Microarray Suite 5.0 algorithm (Affymetrix) and raw fold-change for each transcript was calculated by taking the simple ratio of the geometric means of the signal values for each respective group.
|
| |
|
| Submission date |
Feb 29, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Imara Perera |
| E-mail(s) |
[email protected]
|
| Phone |
919-515-3544
|
| Fax |
919-515-3436
|
| Organization name |
North Carolina State University
|
| Department |
Plant Biology
|
| Street address |
Campus Box 7612
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10670 |
Global expression profiling of wild type and transgenic Arabidopsis plants in response to water stress |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
