|
| Status |
Public on Nov 08, 2017 |
| Title |
HL60 BCAT1-OE -DOX_1_10weeks |
| Sample type |
genomic |
| |
|
| Source name |
HL-60 CTRL_10weeks
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HL-60
genotype/variation: CTRL
|
| Treatment protocol |
Doxycyclin 10μg/ml
|
| Growth protocol |
RPMI + 10% FCS (medium change every 3 days)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified using Qiagen QiAMP Kit according to standard instructions
|
| Label |
Biotin, Cy3-Streptavidin
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Gene Expression Microarrays were scanned using the Illumina iScan-Scanner according to Standard Illumina Scanning Protocol
|
| Data processing |
The data were normalised for red and green channel seperately using quantile normalisation with R using function normalize.quantiles from Bioconductor package preprocessCore
|
| |
|
| Submission date |
Jul 04, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Simon Raffel |
| E-mail(s) |
[email protected]
|
| Organization name |
German Cancer Research Center
|
| Street address |
INF 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE100779 |
Role of Branched Chain Amino Acid Transaminase 1 (BCAT1) in Acute Myeloid Leukemia [methylation_HL60_BCAT1-OE_10weeks] |
| GSE100784 |
Role of Branched Chain Amino Acid Transaminase 1 (BCAT1) in Acute Myeloid Leukemia |
|
