|
| Status |
Public on Sep 25, 2008 |
| Title |
dor-ck-1, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
leaf of dor mutant under control
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: arabidopsis Columbia, SALK T-DNA insertion (Salk-050074)
age: dor plants were grown under normal watering conditions for 34 days.
|
| Growth protocol |
dor plants were grown under normal watering conditions for 34 days. Arabidopsis rosette leaves samples were homogenized in liquid nitrogen prior to RNA isolation
Arabidopsis plants were grown in soil under long-day conditions (16 hr light; 8 hr dark) at 22 degree
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated by RNAplant reagent ((Tiangen Biotech))and purified by Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Arabidopsis ATH1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from leaf of S29 mutant under control condition
|
| Data processing |
The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Feb 26, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Wenying Xu |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
|
| Street address |
Datun Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10643 |
Transcription profiling of Arabidopsis dor mutant and wild-type plants in response to drought stress. |
|
| Relations |
| Reanalyzed by |
GSE119083 |
