|
| Status |
Public on Mar 01, 2008 |
| Title |
EGF2, chip MOE430A |
| Sample type |
RNA |
| |
|
| Source name |
EGF-responsive neural stem cell (E14), E14 ge
|
| Organism |
Mus musculus |
| Characteristics |
Strain: CD-1
Gender: mixed
Karyotype: normal
Defining markers: PDGFR-alpha
|
| Growth protocol |
Growth medium: DMEM:F12 N2
Media supplements: 2% B27
Culture conditions: PDGF-AA 100ng/ml, 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep method: RNeasy
Amplification method: none
Cell purification method: Isolation based on neurosphere formation in response to EGF
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.
|
| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Samuel Weiss ([email protected]; ) for analysis.
Stembase Experiment ID: E237
Stembase Experiment ID link: http://www.stembase.ca/?path=/browse/experiment&id=237
SCGP Sample ID: S330
SCGP Sample ID link: http://www.stembase.ca/?path=/browse/experiment&id=237#SAMPLE_368
Short description: Seven day old epidermal growth factor-generated neurospheres cultured from the embryonic day 14 basal ganglia (medial and lateral ganglionic eminences).
RNA concentration: 100ng/ul
Sample volume: 25-30ul
|
| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpha2=0.06
|
| |
|
| Submission date |
Feb 22, 2008 |
| Last update date |
Feb 29, 2008 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
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