|
| Status |
Public on Jul 19, 2017 |
| Title |
H9 ESC-CM Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Exosome H9 R2
|
| Organism |
Homo sapiens |
| Characteristics |
source: Media supernatant
|
| Treatment protocol |
CMs were grown in RPMI-1640 media supplemented with B27 supplement minus antioxidants for 36 hours, following which the supernatant was removed and collected for exosome isolation.
|
| Growth protocol |
Day 30 ESC-CMs and iPSC-CMs were cultured routinely
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Exiqon miRCURY RNA Isolation Kit
|
| Label |
Cy3
|
| Label protocol |
Arraystar Flash RNA Labeling Kit was used for sample labeling
|
| |
|
| Hybridization protocol |
Hybridization was performed in Agilent SureHyb Hybridization Chambers
|
| Scan protocol |
Slides were scanned with Agilent DNA Microarray Scanner
|
| Description |
LncRNA profiling of exosomes collected at 36 hours at normoxia
|
| Data processing |
Data were extracted using Agilent Feature Extraction software
|
| |
|
| Submission date |
Jun 20, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Joseph Wu |
| Organization name |
Stanford University School of Medicine
|
| Street address |
Stanford University School of Medicine
|
| City |
Stanford |
| State/province |
Carlifornia |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL21827 |
| Series (1) |
| GSE100218 |
Comparison of non-coding RNAs in exosomes and functional efficacy of human embryonic stem cell- versus induced pluripotent stem cell-derived cardiomyocytes |
|
