|
| Status |
Public on Nov 07, 2008 |
| Title |
chondrocyte wild type-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
wild type mouse primary chondrocyte
|
| Organism |
Mus musculus |
| Characteristics |
Strain:129/C57B6, tissue:bone
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Sepasol-RNA I (Nacalai Tesque). Qulity of total RNA was checked using a bioanalyzer (Agilent).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed using One-cycle target labeling kit (Affymetrix) as manufacture's indication.
|
| |
|
| Hybridization protocol |
Twenty micro gram of labeled sample was used. Hybridization was performed at 45C for 16hr with continuous rotation.
|
| Scan protocol |
Washing and streptavidin-phycoerythrin staining was done using a GeneChip Fluidics Station (Affymetrix), then scanning was performed using a GeneChip scanner 3000 (Affymetrix) as the default setting.
|
| Description |
Primary chobdrocytes were isolated and cultured as described previously (Lefebvre V., et al. Matrix Biol. 14, 329-335, 1994).
|
| Data processing |
CHP file was directly submitted to GEO. MAS 5.0 algorithm
|
| |
|
| Submission date |
Feb 19, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Toshiyuki Fukada |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10556 |
Comparision of expression profile between wild-type and Slc39a13 knockout chondrocytes |
|
| Relations |
| Reanalyzed by |
GSE119085 |
