|
| Status |
Public on Nov 13, 2018 |
| Title |
Homeostatic epidermis IFE cells in G9a WT, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Homeostatic epidermis interfollicular cell in G9a WT
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Epidermis
genotype: G9a WT
age: 15-week old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRizol (Sigma-Aldrich).
|
| Label |
biotin
|
| Label protocol |
cDNA library preparation and amplification were performed from 25ng total RNA using WTA2 (Sigma-Aldrich) with 17cycles of amplification. 8µg cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 10Kv2 Assay Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridisation mixture was prepared according to the Gene Atlas protocol (Affymetrix). Each sample target was hybridised to a Mouse Genome 430 PM array. After hybridisation for 16 hours at 45°C, washing and staining was performed in the GeneAtlas Fluidics Station (Affymetrix).
|
| Scan protocol |
The arrays were scanned in a GeneAtlas Imaging Station (Affymetrix). All processing was performed according to manufacturer’s recommendations.
|
| Data processing |
Overall array intensity was normalised between arrays and the probe intensity of all probes in a probe set summarised to a single value using RMA (Robust Multichip Average)
|
| |
|
| Submission date |
Jun 12, 2017 |
| Last update date |
Nov 13, 2018 |
| Contact name |
Aikaterini Symeonidi |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut for Research in Biomedicine (IRB-Barcelona)
|
| Department |
Oncology
|
| Lab |
Stem cells and Cancer Lab
|
| Street address |
Baldiri i Reixac, 10
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE99934 |
Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours |
| GSE99956 |
Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours |
|
