|
| Status |
Public on Feb 21, 2008 |
| Title |
BAF250 knock-out ES cells, ko2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse total RNA of ko2, BAF250 knock-out homozygous ES cells
|
| Organism |
Mus musculus |
| Characteristics |
knock-out ES cell lines are derived in vitro from wild type E14g ES cell line.
|
| Growth protocol |
ES cells are cultured in gelatin wells with feeder free ES cell medium: 1 x GMEM medium supplemented with 2 mM glutamine, 1 mM sodium pyruvate,1 x nonessential amino acids,10% (v/v) fetal bovine serum,a 1:1000 dilution of beta-mercaptoethanol stock solution, and 500-1000 units per ml of leukocyte inhibitory factor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip mouse genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner.
|
| Description |
Gene expression data from BAF250 knock-out homozygous ES cells compared to wild type ES cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ping Hu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, Berkeley
|
| Department |
MCB
|
| Lab |
Tjian
|
| Street address |
Hearst & Oxford
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94564 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
