|
| Status |
Public on Feb 14, 2008 |
| Title |
normal donors TA1 |
| Sample type |
RNA |
| |
|
| Source name |
normal donors
|
| Organism |
Homo sapiens |
| Characteristics |
blood-derived macrophages
|
| Biomaterial provider |
HSS
|
| Treatment protocol |
no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Labeled cDNA was prepared with BioArray High Yield RNA Transcription Kit (Enzo Life Sciences, Farmingdale,NY)
|
| |
|
| Hybridization protocol |
Samples from healthy donors were pooled before hybridization.
Then hybridized to Affymetrix HG U95 Av2 GeneChip using Affymetrix fluidics station according to the instructions of the manufacturer
|
| Scan protocol |
Affymetrix Gene-Array Scanner
|
| Description |
blood-derived macrophages from healthy donors
|
| Data processing |
Affymetrix Microarray Suite MAS 5.0. Images were scaled to an average hybridization intensity of 250. Further data analysis was performed using GeneSpring software.
|
| |
|
| Submission date |
Feb 12, 2008 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Taras Antoniv |
| Organization name |
Hospital for Special Surgery
|
| Department |
Research
|
| Lab |
Lionel B. Ivashkiv, MD
|
| Street address |
535 East 70th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE10500 |
Gene expression in rheumatoid arthritis synovial macrophages |
|
