|
| Status |
Public on Feb 06, 2008 |
| Title |
MPA 3 |
| Sample type |
RNA |
| |
|
| Source name |
CD1, E15 pregant mouse
|
| Organism |
Mus musculus |
| Characteristics |
MPA-treatment for 48hrs
|
| Treatment protocol |
MPA (1mg/dam) SubQ injection for 48 hours; or the same volume of EtOH
|
| Growth protocol |
CD1 mice on gestation of day 15
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Qiagen RNeasy kit purification
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (WT-Ovation Pico RNA Amplification System, NuGEN Technologies).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.2 ug of cDNA were hybridized for 16 hr at 45C on GeneChip mouse Genome 430V2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 7G.
|
| Description |
gene expression data from pregnant mouse cervix treated with MPA
|
| Data processing |
gcrma calculated from cel files using Stratagene ArrayAssist Lite v3.4, producing chp files with unlogged signal levels and Affy flags
|
| |
|
| Submission date |
Feb 05, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Hua Xu |
| E-mail(s) |
[email protected]
|
| Phone |
215-746-6865
|
| Organization name |
University of Pennsylvania
|
| Department |
OB/GYN
|
| Lab |
1349 BRB II/III
|
| Street address |
421 Curie Blvd
|
| City |
philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10392 |
Gene regulation profile of Medroxyprogesterone acetate (MPA)-treated late pregnant cervix |
|
| Relations |
| Reanalyzed by |
GSE119085 |
