|
| Status |
Public on Mar 01, 2008 |
| Title |
healthy CD4 T cells 2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood from healthy individuals
|
| Organism |
Homo sapiens |
| Characteristics |
healthy donor
|
| Treatment protocol |
PBMC were isolated, tagged with fluorochrome-conjugated antibodies to CD3, CD4, CD19 and CD33 for 30 min. PBMC were sorted into subsets on a Cytomation MoFlo cytometer into individual subsets. Purity of the populations was confirmed after sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini column; only high quality RNA samples as assessed by BioAnalyzer were hybridized to the GeneChips
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Affymetrix protocol from at least 5 ug of high quality RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, hybridization was carried out according to the Affymetrix Expression Analysis Technical Manual; only samples passing stringent quality control guidelines were assessed
|
| Scan protocol |
Affymetrix arrays were scanned using the Affymetrix GeneChip Scanner 3000 System
|
| Description |
Gene expression data from freshly sorted cells
|
| Data processing |
Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) software using the Affymetrix default analysis settings and global scaling normalization methods.
|
| |
|
| Submission date |
Jan 30, 2008 |
| Last update date |
Apr 05, 2011 |
| Contact name |
Laurie S Davis |
| E-mail(s) |
[email protected]
|
| Phone |
214-648-2201
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Internal Medicine
|
| Lab |
Davis
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8884 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE10325 |
Expression data from human peripheral blood subsets |
|
