|
| Status |
Public on Jun 20, 2017 |
| Title |
WT_Colon_MF_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Colon, isolated MF, Replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue specific cells: Colon macrophages
gender: male
age: 6 weeks
|
| Treatment protocol |
Cells were isolated ex vivo and cells were directly isolated within a total range of time of 5h.
|
| Growth protocol |
Different macrophage populations were freshly isolated from WT mice (C57BL6/J) grown in SPF conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The miRNeasy total RNA extraction kit (Qiagen) was used for RNA isolation.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled miRNA was prepared from 100ng total RNA using Agilent miRNA Complete Labeling and Hyb Kit according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized with a 8X15 mouse miRNA array rel v19 (design number 046065) at 55°C and 20 rpm for 20 hours, and then washed according to the manufacturers instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on Agilent Technologies Scanner G2505B US22502692 using 5uM resolution.
|
| Description |
miRNA expression in colon macrophages
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol miRNA_107_Sep09 and Grid: 029152_D_F_20100702) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Apr 25, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Steffen Jung |
| E-mail(s) |
[email protected]
|
| Phone |
0097289342787
|
| Organization name |
The Weizmann Institute of Science
|
| Department |
Immunology and Regenerative Biology
|
| Lab |
Steffen Jung
|
| Street address |
Herzl
|
| City |
REHOVOT |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL17912 |
| Series (2) |
| GSE98143 |
Differential impact of Dicer deficiency on microglia of the developing and adult brain (Agilent miRNA microarrays) |
| GSE98144 |
Differential impact of Dicer deficiency on microglia of the developing and adult brain |
|
