|
| Status |
Public on Jan 04, 2008 |
| Title |
PBMCs-Type 1 Diabetes-IDDM4_T1D_New_B |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs from Type 1 Diabetes patient and the timepoint at diagnosis.
|
| Organism |
Homo sapiens |
| Characteristics |
Age: 10 yrs, when sample taken.
Gender: F
Ethnicity: Hispanic or Latino
Race: Selected More than One Race
Illness: Type 1 Diabetes
Treatment: Insulin
pH<7.30 at time of diagnosis: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
EukGE-WS2v5
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.
|
| Description |
Type 1 Diabetes patient and the timepoint at diagnosis.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jan 03, 2008 |
| Last update date |
Jan 03, 2008 |
| Contact name |
Ellen Grishman |
| E-mail(s) |
[email protected]
|
| Organization name |
UTSW Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE9006 |
Gene expression in PBMCs from children with diabetes |
|
