|
| Status |
Public on Jul 01, 2008 |
| Title |
AML P45 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA isolated bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
DNA isolated from bone marrow of a patient with AML M1, Age: 69 Gender:female
lab code: P45
diagnosis: AML M1
age: 69
sex: f
tissue: BM
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from bone marrow using the Wizard Genomic DNA exraction kit from Promega
|
| Label |
Cy3
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| Channel 2 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
DNA isolated from the blood of a healthy female donor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by a salting out procedure (Blin and Stafford,1976).
|
| Label |
Cy5
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| |
| Hybridization protocol |
Hybridization was done overnight at 42C in a slide booster (Advalytix, Brunnthal, Germany). Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| Scan protocol |
Slides were scanned using an Agilent scanner .
|
| Description |
Array CGH analysis of a patient with AML
|
| Data processing |
TIFF images were analysed by Genepix 5.0 (Axon Instruments, Union City, CA) and raw intensities (gpr files) were imported into CGHPRO (Chen at al.,2005). Background intensities were not substracted. Signal intensities were normalized by subgridd LOWESS. Aberrant ratios involving three or more neighbouring clones wereconsidered as genomic aberrations, unless they coincided with a known polymorphism as listed in the Database of Genomic Variants (version Dec 2005)
|
| |
|
| Submission date |
Dec 18, 2007 |
| Last update date |
Nov 18, 2008 |
| Contact name |
Reinhard Ullmann |
| E-mail(s) |
[email protected]
|
| Phone |
00493084131251
|
| Organization name |
MPIMG
|
| Department |
Human Molecular Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
Ihnestr.73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5000 |
| Series (1) |
| GSE9928 |
Analysis of AML/MDS patients with 11q/MLL amplification |
|
