|
| Status |
Public on Oct 11, 2019 |
| Title |
GTF2IRD2 inducible clone, biological replicate3 [hRD2_A5_T24] |
| Sample type |
RNA |
| |
|
| Source name |
inducible ES stable cell line grown in medium deprived of Tetracycline
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse Embryonic Stem (ES) cells
genotype/variation: inducible transgenic line overexpressing the human isorofm of the gene GTF2IRD2
|
| Growth protocol |
The growth of each inducible clone was performed in medium deprived of Tetracycline for 24 hours, while the control clones were grown in medium containing Tetracycline and the total RNA was extracted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs from either undifferentiated ES cells were purified by QIAshredder (Qiagen spa, Milano, Italy, catalog no. 79656) and extracted with RNeasy Protect Mini Kit (Qiagen, catalog no. 74126) using standard conditions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cRNAs were generated using the GeneChip IVT labelling kit (Affymetrix). 20µg of Biotinylated cRNA was fragmented and 10µg hybridised to the Affymetrix GeneChip Mouse Genome 430_2 array for 16hrs at 45°C using an Affymetrix GeneChip Fluidics Station 450 according to the manufacturer’ standard protocols.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 .
|
| Description |
SAMPLE 6
Gene expression data from mouse inducible ES stable clone
|
| Data processing |
The data were analyzed with the Bioconductor package Affy using RMA method for normalizing and summarizing probe level intensity measurements.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
Oct 13, 2019 |
| Contact name |
Rossella De Cegli |
| E-mail(s) |
[email protected]
|
| Phone |
08119230692
|
| Organization name |
Tigem
|
| Street address |
Via Campi Flegrei 34
|
| City |
Pozzuoli |
| ZIP/Postal code |
80078 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE95268 |
Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2IRD2 |
| GSE96701 |
A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem |
|
