|
| Status |
Public on Feb 16, 2017 |
| Title |
RKO WT siCKIa 3 |
| Sample type |
RNA |
| |
|
| Source name |
RKO cell line P53 Wildtype + CK1a knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RKO cell line P53 Wildtype
treatment: Cells treated with CK1a siRNA (100nM)
genotype/variation: P53 Wildtype + CK1a knockdown
|
| Treatment protocol |
Isogenic cells were transfected with either CK1a or negative control siRNA at an end concentration of 100nM. Cells were harvested 48hrs after transfection after confirmation of CK1a knockdown by WB
|
| Growth protocol |
P53 isogenic RKO cells(+/+ and -/-) were cultured in 6 well plates at 37 degrees celsius with McCoy medium supplemented with 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the miRNeasy kit (Qiagen) according to the given protocol
|
| Label |
Cy3
|
| Label protocol |
Standard Agilent labeling protocol
|
| |
|
| Hybridization protocol |
miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol
|
| Scan protocol |
Slides were scanned using the SureScan Microaarray scanner with a scan resolution of 3uM
|
| Description |
Chip ID: 254606412303_1_2
RKO cell line P53 Wildtype + CK1a knockdown, biological replicate 3
|
| Data processing |
Scanned images were analyzed with Feature Extraction Software FE version 9.5.3 (Agilent)
|
| |
|
| Submission date |
Feb 15, 2017 |
| Last update date |
Feb 16, 2017 |
| Contact name |
Mohammed Abba |
| E-mail(s) |
[email protected]
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Department |
Molecular Oncology of Solid Tumors
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
D-69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19730 |
| Series (1) |
| GSE94908 |
P53 regulated microRNAs in the presence/absence of caesin kinase 1 alpha (CK1alpha) |
|
