|
| Status |
Public on Dec 11, 2007 |
| Title |
normal brain 86N (50K_Xba240_SNP) |
| Sample type |
genomic |
| |
|
| Source name |
normal brain
|
| Organism |
Homo sapiens |
| Characteristics |
normal brain tissue
gender: M
age: 60
survival (days):439
|
| Treatment protocol |
Frozen tissue was lysed and DNA purified using the Qiagen DNeasy kit
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA was performed according to the Qiagen DNeasy kit instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA were prepared according to the standard Affymetrix protocol from 250 ng genomic DNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 40 ug of cRNA were hybridized for 16 hr at 48C on GeneChip 100K Hind Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
SNP array data from a normal brain
|
| Data processing |
Signal intensity values were obtained with dChip using invariant set normalization.
Genotypes were determined by Affymetrix Genotyping Tools Version 2.0.
|
| |
|
| Submission date |
Dec 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Rameen Beroukhim |
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL2005 |
| Series (2) |
| GSE9635 |
Assessing the significance of chromosomal aberrations in cancer: Methodology and application to glioma |
| GSE14860 |
Integrated genomic profiling of endometrial carcinoma |
|
