|
| Status |
Public on Apr 01, 2008 |
| Title |
Col-0-SH-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
7-day old, Col-0 treated with 1hr of low R:FR light
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Ecotype: Col-0
Age: 7 days
Tissue: whole seedling
|
| Biomaterial provider |
Yi Tao, Plant Biology Laboratory, The Salk Institute
|
| Treatment protocol |
Seeds were surface sterilized and plated on 1/2 Murashige Skoog (MS) medium Seedlings were grown at 22 degree, under simulated continuous white light, mixture of Red: 13 µ mol m-2 s-1, Blue (B): 1.23 µ mol m-2 s-1 for 7-day, then treated with low R:FR light (mixture of Red: 13 µ mol m-2 s-1, Blue (B): 1.23 µ mol m-2 s-1,FR: 20.2 µ mol m-2 s-1 )for 1hr
|
| Growth protocol |
Seedlings were grown at 22 degree, under simulated continuous white light, mixture of Red: 13 µ mol m-2 s-1, Blue (B): 1.23 µ mol m-2 s-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spectrum™ Plant Total RNA Kit (Sigma)
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
The purpose of this experiment is to study the underlying molecular mechanism that leads to the different responses of Col-0, sav3 and sav1-1 to shade.
|
| Data processing |
All 18 arrays were processed using gcRMA method provided by bioconductor
|
| |
|
| Submission date |
Dec 07, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joanne Chory |
| E-mail(s) |
[email protected]
|
| Phone |
858-453-4100
|
| URL |
http://cactus.salk.edu
|
| Organization name |
The Salk Institute for Biological Studies
|
| Department |
Plant Biology Laboratory
|
| Street address |
10010 N. Torrey Pines Road.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE9816 |
Gene expression profiling of sav mutants |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
