|
| Status |
Public on May 25, 2017 |
| Title |
bladder_cancer_turbt_sample_73 |
| Sample type |
RNA |
| |
|
| Source name |
FFPE bladder cancer
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: muscle-invasive bladder cancer (MIBC)
treatment: pre-NAC
sample_type: transurethral resection of bladder tumor (TURBT)
age: NA
cstage: T3
cnode: 0
bca_subtype: Basal
|
| Treatment protocol |
Not Applicable.
|
| Growth protocol |
Not Applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After pathologic review, tissue was harvested from formalin-fixed, paraffin-embedded tumor blocks by 1mm punch under RNase-free conditions. Total RNA was extracted and purified using RNeasy FFPE kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and biotinylated using the WT-Ovation FFPE system (NuGen, San Carlos, CA) according to the manufacturer’s recommendations with minor modifications.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials and protocols.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
GNDX.NAC73
bca_subtype=bladder cancer subtype
|
| Data processing |
Data was normalized using SCAN (SCAN.UPC package from Bioconductor).
|
| |
|
| Submission date |
Jan 26, 2017 |
| Last update date |
Feb 10, 2019 |
| Contact name |
Elai Davicioni |
| E-mail(s) |
[email protected]
|
| Organization name |
GenomeDx
|
| Street address |
1152 Mainland St #430
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6B 4X2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL22995 |
| Series (1) |
| GSE87304 |
Impact of molecular subtypes in muscle-invasive bladder cancer on predicting response and survival outcome to neoadjuvant chemotherapy: results from a multi-institutional validation study |
|
