|
| Status |
Public on Jul 21, 2017 |
| Title |
Young normal diet liver, ZT 4, replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
liver_YND_ZT4_5
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: C57BL6/J
age: 50 weeks
diet: Normal diet
zt: 4
|
| Treatment protocol |
Mice were fed either a normal diet or calorie-restricted diet for 25 weeks
|
| Growth protocol |
Animals were housed in 12 hour Light/Dark conditions and harvested livers every 4 hours over the circadian cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRizol (Life Technologies)
|
| Label |
biotin
|
| Label protocol |
cDNA library preparation and amplification were performed from 25ng total RNA using WTA2 (Sigma-Aldrich) with 17 cycles of amplification. 8µg cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 10Kv2 Assay Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridisation mixture was prepared according to the Gene Atlas protocol (Affymetrix). Each sample target was hybridised to a Mouse Genome 430 PM array. After hybridisation for 16 hours at 45°C, washing and staining was performed in the GeneAtlas Fluidics Station (Affymetrix).
|
| Scan protocol |
The arrays were scanned in a GeneAtlas Imaging Station (Affymetrix). All processing was performed according to manufacturer’s recommendations.
|
| Description |
Gene expression data from mouse liver at indicated ZT
|
| Data processing |
Overall array intensity was normalised between arrays and the probe intensity of all probes in a probe set summarised to a single value using RMA (Robust Multichip Average). By selecting the probe that has the highest interquantile range per gene, we reduce the probeset to one probe per gene.
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| |
|
| Submission date |
Jan 21, 2017 |
| Last update date |
Jul 21, 2017 |
| Contact name |
Shogo Sato |
| E-mail(s) |
[email protected]
|
| Organization name |
Texas A&M University
|
| Department |
Biology
|
| Street address |
3258 TAMU, Biological Science Building West (BSBW) 309
|
| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77843-3258 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE93903 |
Impact of aging/caloric restriction on circadian gene expression |
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