|
| Status |
Public on Dec 07, 2007 |
| Title |
middle flower buds of J03B, biol_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
middle flower buds, fertile plants, J03
|
| Organism |
Brassica napus |
| Characteristics |
Heterozygous two-type line J03B, Genotype: msmsrfrf
flower buds from 1mm to 3mm that contain tetrad stage and uninucleate stage
|
| Treatment protocol |
Flower buds were isolated from B. napus and were dissected on intact plants under the same growth conditions from the same field. The flower buds that are <1, 1, 2, 3 and >3 mm were harvested. All harvested samples including stamens and pistils were removed and immediately (<2 sec) frozen in liquid nitrogen to minimize post-harvest changes in gene expression. Flower buds were collected from at least two different biological repetitions within each line, from between two and four inflorescences on each plant, and from between two and four different plants from each line. Different flower bud samples were collected at approximately the same time of the day to avoid diurnal effects on gene expression.
|
| Growth protocol |
Plants were prepared by growing the seeds in the rapeseed research field of Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from flower buds using Trizol reagent following instructions from the manufacturer (Invitrogen Corporation, Carlsbad, CA, USA), and was then purified on RNeasy columns (Qiagen, Valencia, CA, USA). Total RNA with an absorbance A260/A280 ratio >1.8 was used for further analysis.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Arabindopsis ATH-1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from floura during anther abortion
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using User Defined analysis settings and global scaling as normalization method. Normalization value was set to 1 and the target signal was set to 500.
|
| |
|
| Submission date |
Nov 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
hua cai dong |
| E-mail(s) |
[email protected]
|
| Phone |
86+027-86829403
|
| Organization name |
Institute of Oil Crops Research of CAAS
|
| Department |
Genomics and Molecular Biology
|
| Lab |
Functional genomics
|
| Street address |
Xudong Second Road No2
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430062 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE9721 |
Expression data from flowers of two isogenic lines J10AB and J03AB at different developmental stages |
|
| Relations |
| Reanalyzed by |
GSE119083 |
