strain: Wistar Kyoto (WKY) stress: diesel protocol: Wistar Kyoto (WKY) male rats (12-14 wks age) exposed whole body to 2.0 mg/m3 diesel emission particles for 6h/d, 5 d/wk for 4 wks. tissue: left ventricular heart
Extracted molecule
total RNA
Extraction protocol
Qiagen RNeasy columns
Label
biotin
Label protocol
Affymetrix labeling protocol
Hybridization protocol
Affymetrix hybridization protocol
Scan protocol
Affymetrix GCOS scanning protocol
Description
Diesel emision particle exposure is linked to increases in cardiovascular effects. This effect is enhanced in individuals with pre-existing disease. Animal models of cardiovascular disease are used to study this susceptibility. The heart is rich in mitochondria, which produce high levels of free radicals, leading to inactivation of tricarboxylic acid cycle enzymes. We hypothesized that a 4-wk DEP inhalation would result in strain-related structural impairment of cardiac mitochondria and changes in these enzyme activities in WKY and SHR. Male rats (12-14 wks age) were exposed whole body to air or 0.5 or 2.0 mg/m3 DEP for 6h/d, 5 d/wk for 4 wks.
