|
| Status |
Public on Feb 29, 2008 |
| Title |
Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep1_exp3 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis leaves 4 week-old plants from Ws-4, wild-type, inoculated with Xcc147 strain, at 108 cfu/ml, pool of 90-105 min harvest
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Genotype : Ws-4
Transformation : no
Age : leaves of 4 week-old plants
Bacterial inoculation : Xcc147 at 108 cfu/ml
Harvest : 90-105 min post inoculation
|
| Treatment protocol |
Leaves of 4 week-old plants were syringe-infiltrated in totality, with the Xcc147 strain, at 108 cfu/ml, harvested at various times after inoculation, pooled and frozen
|
| Growth protocol |
Arabidopsis plants were grown under controlled conditions as previously described (Lacomme and Roby, 1996, Plant. Mol. Biol., 30, 995-1008)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted as described by Lorrain et al., 2004, Plant cell, 16, 2217-2232
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45°C on ATH1 Arabidopsis GeneChip. GeneChips were washed and stained in the Affymetrix GenChip Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GenChip scanner 3000
|
| Description |
Gene expression data from Arabidopsis leaves 4 week-old plants from Ws-4, wild-type, inoculated with Xcc147 strain, at 108 cfu/ml, pool of 90-105 min harvest
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
|
| |
|
| Submission date |
Nov 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dominique ROBY |
| E-mail(s) |
[email protected]
|
| Phone |
33 05 61 28 55 11
|
| Fax |
33 05 61 28 50 61
|
| Organization name |
CNRS/INRA
|
| Lab |
Laboratoire des Interactions Plantes - Microorganismes
|
| Street address |
Chemin de Borde Rouge
|
| City |
Castanet-Tolosan |
| ZIP/Postal code |
F-31326 |
| Country |
France |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE9674 |
Expression data from Arabidopsis plants misexpressing AtMYB30 after Xanthomonas inoculation at early timepoints |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
