|
| Status |
Public on Jan 01, 2017 |
| Title |
Shoots of WT plants after iron withdrawal for 3 days, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Shoots of 13 day old seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Shoots
developmental stage: 13 day old seedlings
treatment: Medium prepared without iron
genotype: Columbia (Col0) wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy plant
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to Affymetrix 3’ IVT Express Kit Manual from 500ng of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arabidopsis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G Scanner.
|
| Description |
Gene expression data from shoots of WT plants after iron withdrawal for 3 days
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Dec 21, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Elsbeth Lewis Walker |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Massachusetts
|
| Department |
Biology Department
|
| Lab |
Elsbeth Walker
|
| Street address |
611 North Pleasant St
|
| City |
Amherst |
| State/province |
Massachusetts |
| ZIP/Postal code |
01003 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE92716 |
Iron_deficiency gene expression in Arabidopsis wild type and yellow stripe1-like1/3 (ysl1ysl3) double mutants. |
|
