|
| Status |
Public on May 30, 2017 |
| Title |
copt5 2 Cu 1uM |
| Sample type |
RNA |
| |
|
| Source name |
ko copt5_Cu 1 uM
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype background: columbia 0
genotype/variation: copt5-2 knockout mutant
age: 7 days
tissue: whole seedlings
growth condition: 1/2 MS + 1uM Cu (control condition)
|
| Growth protocol |
Arabidopsis thaliana columbia 0 as wt and copt5-2 mutant (knockout mutant T-DNA insertion) seeds were grown during 7 days. The growth medium was 1/2 MS, supplemented by Cu 1uM in the control conditions and by BCS 100uM for the treatment, in order to generate severe Cu defficiency. Light conditions were DHLC(12/12) and the material was collected after 12 hours of light. 3 biological replicates with 4 samples of each one were collected (in total 12 samples).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quiagen RNeasy Plant Mini Kit was employed to obtain total RNA from samples studied. The extraction protocol followed the manufacturer instructions
|
| Label |
Ko 2 B
|
| Label protocol |
Label protocol was performed by the technical service in the IBMCP (Politecnic University of Valencia) according to the manufacturer protocol of Agilent microarray system
|
| |
|
| Hybridization protocol |
Hybridization protocol was performed by the technical service in the IBMCP (Politecnic University of Valencia) according to the manufacturer protocol of 4x44 Agilent microarray system
|
| Scan protocol |
Samples were scanned by an Axon Instruments Genepix 4000B
|
| Description |
ko 2 B
|
| Data processing |
Results obtained were processed by Genespring GX software academic license
|
| |
|
| Submission date |
Dec 08, 2016 |
| Last update date |
May 30, 2017 |
| Contact name |
Angela Carrió-Seguí |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Valencia
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
Dr. Moliner 50
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
46100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE91044 |
The vacuolar high affinity copper transporter COPT5 participates in the copper requirement for iron signaling in Arabidopsis Thaliana |
|
