|
| Status |
Public on Oct 22, 2016 |
| Title |
Benign basal cells from patient 1 |
| Sample type |
RNA |
| |
|
| Source name |
Benign human prostate patient 1
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: patient 1
tissue: Benign human prostate
cell subpopulation: Benign basal cells
|
| Treatment protocol |
Tissues were dissociated to single cells using standard protocols described in Goldstein et al, Nature Protocols, 2011
|
| Growth protocol |
Tissue was collected from UCLA's Translational Pathology Core Laboratories within 24 hours of patients undergoing radical prostatectomy at UCLA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from sorted cells using the RNeasy Micro kit (QIAGEN) and amplified with the NuGEN Pico kit according to standard manufacturer protocols.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared from total RNA using the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Biotinylated cDNA was hybridized to the Affymetrix Human Genome U133 Plus 2.0 Chip
|
| Scan protocol |
Chips were scanned using an Affymetrix GeneChip Scanner 7G
|
| Description |
BE BAS 1
Gene expression data from basal cells isolated from benign human prostate
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
affymetrix-algorithm-name = ExpressionStat
affymetrix-algorithm-version = 5.0
program-name = Expression Console
program-id = 1.2.0.20
|
| |
|
| Submission date |
Oct 21, 2016 |
| Last update date |
Oct 22, 2016 |
| Contact name |
Andrew Scott Goldstein |
| Organization name |
UCLA
|
| Department |
Urology
|
| Lab |
Goldstein
|
| Street address |
610 Charles E Young Dr East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE89050 |
Gene expression profiling of three human prostate epithelial subpopulations |
|
