|
| Status |
Public on Oct 20, 2017 |
| Title |
let7_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
P19 cells
|
| Organism |
Mus musculus |
| Characteristics |
condition: let-7a overexpression
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed with miRNeasy columns (Qiagen), according to manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
RNA samples were labeled with Cy3 dye using the low RNA input QuickAmp kit (Agilent)
|
| |
|
| Hybridization protocol |
Agilent : 600.0 ng at 65 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner
|
| Data processing |
Feature extraction data processing. Quantile normalization and differential analysis using Limma package in R / Bioconductor
|
| |
|
| Submission date |
Oct 21, 2016 |
| Last update date |
Oct 20, 2017 |
| Contact name |
Silvia Bottini |
| E-mail(s) |
[email protected]
|
| Organization name |
C3m
|
| Street address |
299, chemin de la ginestiere
|
| City |
Nice |
| ZIP/Postal code |
06200 |
| Country |
France |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE89023 |
Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq [Agilent] |
| GSE89033 |
Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq |
|
