|
| Status |
Public on Sep 30, 2016 |
| Title |
Social defeat - susceptible 3 |
| Sample type |
RNA |
| |
|
| Source name |
Ago2-Amygdala IP following social defeat
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Mouse brain amygdala
strain: C57BL/6J
|
| Treatment protocol |
Amygdala samples were collected from social defeat and control mice 8 days after the end of the chronic social defeat protocol
|
| Growth protocol |
10-week old C57BL/6J male mice were subjected to a chronic social defeat protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy kit, miRNA supplementary protocol (QIAGEN, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
100ng of Total RNA were used. cDNA samples were generated and labeled using Ambion WT Expression Kit and Affymetrix GeneChip WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
2ug ss cDNA in 80 ul were hybridized to a Mouse Gene 1.0 ST array using Affymetrix hybridization kit materials and hybridized for 17 hours at 45° at 60rpm. Post-hyb washes were done with Affymetrix wash solutions on the FS450 station using FS450_0007 protocol.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
total RNA extracted from the Ago2-amygdala complex following an Ago2 IP
Gene expression data from mice Ago2-amygdala following social defeat
|
| Data processing |
RMA was carried out using Partek Genomic Suite 6.5 (Inc. St. Charles, MO; www.partek.com)
|
| |
|
| Submission date |
Sep 29, 2016 |
| Last update date |
Sep 30, 2016 |
| Contact name |
Alon Chen |
| Organization name |
The Weizmann Institute of Science
|
| Department |
Neurobiology
|
| Street address |
234 Herzel St.
|
| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE87489 |
Gene expression data from mice Ago2-amygdala |
| GSE87490 |
Amygdalar microRNA-15a is essential for coping with chronic stress |
|
