|
| Status |
Public on Jan 15, 2008 |
| Title |
102253_CGH |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
GENOMIC DNA REFERENCE
|
| Organism |
Macaca mulatta |
| Characteristics |
sex: female
individual: mmu_313_2000
ancestry: Indian
|
| Biomaterial provider |
New England Primate Research Center
|
| Treatment protocol |
Whole venous blood was collected during routine health evaluations at the New England Primate Research Center. DNA was isolated from peripheral leukoctyes with the Puregene DNA isolation kit (Gentra, Minneapolis, MN) and purified using the Wizard DNA Cleanup System (Promega, Madison, WI).
|
| Growth protocol |
Macaques were housed at the New England Primate Research Center prior to whole venous blood extraction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole venous blood was collected during routine health evaluations at the New England Primate Research Center. DNA was isolated from peripheral leukoctyes with the Puregene DNA isolation kit (Gentra, Minneapolis, MN) and purified using the Wizard DNA Cleanup System (Promega, Madison, WI).
|
| Label |
Cy3
|
| Label protocol |
Sample was labeled and hybridized using standard NimbleGen aCGH protocols in Reykjavik, Iceland. Briefly, 1µg of genomic DNA was labeled with random 9-mer “wobble”-Cy3 or Cy5 (TriLink BioTechnologies, San Diego, CA) using Exo-Klenow DNA polymerase (New England Biolabs, Ipswich, MA), at 37° C for 2 hr.
|
| |
|
| Channel 2 |
| Source name |
GENOMIC DNA TEST
|
| Organism |
Macaca mulatta |
| Characteristics |
sex: female
individual: mmu_353_1991
ancestry: Indian
|
| Biomaterial provider |
New England Primate Research Center
|
| Treatment protocol |
Whole venous blood was collected during routine health evaluations at the New England Primate Research Center. DNA was isolated from peripheral leukoctyes with the Puregene DNA isolation kit (Gentra, Minneapolis, MN) and purified using the Wizard DNA Cleanup System (Promega, Madison, WI).
|
| Growth protocol |
Macaques were housed at the New England Primate Research Center prior to whole venous blood extraction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole venous blood was collected during routine health evaluations at the New England Primate Research Center. DNA was isolated from peripheral leukoctyes with the Puregene DNA isolation kit (Gentra, Minneapolis, MN) and purified using the Wizard DNA Cleanup System (Promega, Madison, WI).
|
| Label |
Cy5
|
| Label protocol |
Sample was labeled and hybridized using standard NimbleGen aCGH protocols in Reykjavik, Iceland. Briefly, 1µg of genomic DNA was labeled with random 9-mer “wobble”-Cy3 or Cy5 (TriLink BioTechnologies, San Diego, CA) using Exo-Klenow DNA polymerase (New England Biolabs, Ipswich, MA), at 37° C for 2 hr.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed using the MAUI Hybridization System (BioMicro Systems, Salt Lake City, UT) at 42º C for 16 hr.
|
| Scan protocol |
Arrays were scanned with a GenePix 4000B 5µm microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
see other fields
|
| Data processing |
Signal intensities were extracted using NimbleScan software (NimbleGen Systems, Inc.), and relative intensity log2 ratios were normalized using the Qspline method.
|
| |
|
| Submission date |
Oct 01, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Arthur S Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Street address |
221 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL5949 |
| Series (1) |
| GSE9220 |
Analysis of CNVs in the rhesus macaque genome identifies candidate loci for evolutionary and human disease studies |
|
