|
| Status |
Public on Sep 26, 2018 |
| Title |
DKO_CMP_1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow, common myeloid progenitor, DKO, replicate1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: bone marrow
cell type: common myeloid progenitor
genotype: Bach1–/–;Bach2–/–
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from sorted common myeloid progenitors by using Rneasy Plus Micro Kit (QIAGEN) and was amplified with an Amino Allyl MessageAmpII aRNA Amplification Kit (Ambion)
|
| Label |
Cy3
|
| Label protocol |
Amplified samples were labeled with indocarbocyanine by using a Cy3 post-labeling reactive dye pack (Amersham)
|
| |
|
| Hybridization protocol |
A sureprint G3 mouse GE microarray slide was used for hybridization according to the manufacturer's protocols (Agilent)
|
| Scan protocol |
After hybridization, a slide was washed adequately and scanned immediately by using an Agilent DNA Microarray Scanner with one color scan setting
|
| Description |
Common myeloid progenitors sorted from bone marrow of DKO
|
| Data processing |
Obtained scan data were analysed by using GeneSpring software (Agilent)
|
| |
|
| Submission date |
Aug 30, 2016 |
| Last update date |
Sep 26, 2018 |
| Contact name |
Kazuhiko Igarashi |
| Organization name |
Tohoku University
|
| Street address |
Seiryo 2-1
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE86253 |
Bach Factors Operate Erythro-Myeloid Differentiation by Responding to Infection |
|
