|
| Status |
Public on Nov 19, 2007 |
| Title |
HCC_KO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HCC of Trim24 KO
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6
Male
Age: 20 months
|
| Growth protocol |
Mice were bred and housed in the Clinical Mouse Institute, ICS (Strasbourg, France) according to guidelines in compliance with the European legislation on care and use of laboratory anaimals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Kit from QIAGEN.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner GCS3000
|
| Description |
Gene expression data from liver tumors of Trim24 KO mice
|
| Data processing |
The data were analyzed with Affymetrix GCOS software version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 11, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Régine Losson |
| E-mail(s) |
[email protected]
|
| Organization name |
IGBMC
|
| Street address |
1 rue Laurent Fries
|
| City |
ILLKIRCH CEDEX |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9012 |
Expression data from spontaneous liver tumors of Trim24/TIF1a-null mice. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
