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Status
Public on May 21, 2004
Title
B chip Version1: time 1 hour Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
235.889
293.956
231.457
228.335
124
181.5
123
120.5
2
-3.78E-01
0
269.553
282.814
264.862
257.535
146
173
140
138
3
7.82E-01
0
1425.95
279.605
872.729
250.177
1144
168
670
148
4
9.93E-02
0
257.725
241.786
236.316
219.399
149
143.5
126
127
5
5.66E-01
0
1168.93
283.331
806.004
222.706
1038
151.5
687
112
6
4.27E-01
0
477.395
253.063
415.069
272.241
331
146
299
154
7
1.01E-01
0
305.007
240.556
282.993
215.908
185
126
170
116
8
1.65E-01
0
301.851
223.176
291.183
233.257
174
118.5
165
127
9
1.21E-01
0
382.821
294.934
332.8
261.073
264
178
204
138
10
5.14E-01
0
265.024
217.646
234.69
245.726
143
125.5
132
133
11
3.09E-01
0
234.014
201.805
250.61
249.738
139
108
148
145.5
12
-7.88E-01
0
237.671
268.73
242.668
262.311
136
172.5
134
138.5
13
-1.07E-03
0
269.417
222.41
280.901
231.125
160
114
155
127
14
-3.80E-01
0
195.577
192.93
254.556
250.489
105
103
147
133
15
-4.41E-01
0
338.37
231.086
453.355
278.546
235
139
310
176
16
-4.45E-01
0
290.604
256.259
347.072
272.921
166
151
224
162.5
17
-7.05E-01
0
349.525
278.689
507.297
294.687
228
157
361
188
18
-3.92E-01
0
254.933
259.897
298.39
270.888
155
149.5
179
158
19
4.81E-01
0
273.188
223.985
266.538
255.757
162
112.5
156
147.5
20
-9.69E-02
0
266.002
235.52
273.233
227.076
157
130
146
126
Total number of rows: 14720 Table truncated, full table size 950 Kbytes .Supplementary data files not provided